Journal: Cureus

Article Title: In Vitro Evaluation of Extracts From Ixora Species for a Potential Phytosomal Formulation

doi: 10.7759/cureus.55396

Figure Lengend Snippet: Antimicrobial activity of petroleum ether, chloroform, ethyl acetate, and hydroalcoholic extracts of Ixora coccinea and Ixora alba leaf and flower ICLPE: Ixora coccinea leaf petroleum ether; ICLCE: Ixora coccinea leaf chloroform extract; ICLEA: Ixora coccinea leaf ethyl acetate; ICLHA: Ixora coccinea leaf hydroalcoholic; ICFPE: Ixora coccinea flower petroleum ether; ICFCE: Ixora coccinea flower chloroform extract; ICFEA: Ixora coccinea flower ethyl acetate; ICFHA: Ixora coccinea flower hydroalcoholic; IALPE: Ixora alba leaf petroleum ether; IALCE: Ixora alba leaf chloroform extract; IALEA: Ixora alba leaf ethyl acetate; IALHA: Ixora alba leaf hydroalcoholic; IAFPE: Ixora alba flower petroleum ether; IAFCE: Ixora alba flower chloroform extract; IAFEA: Ixora alba flower ethyl acetate; IAFHA: Ixora alba flower hydroalcoholic; STD: standard chloramphenicol; + depicts positive; - depicts negative

Article Snippet: Gram-negative strains of Escherichia coli (ATCC-25922), Klebsiella pneumoniae (ATCC-13883), and Pseudomonas aeruginosa (ATCC-27853) were used.

Techniques: Activity Assay

Journal: Microbiology Spectrum

Article Title: Polyproline Peptide Aggregation with Klebsiella pneumoniae Extracellular Polysaccharides Exposes Biofilm Associated Bacteria

doi: 10.1128/spectrum.02027-21

Figure Lengend Snippet: MICs and 50% minimal biofilm eradication concentrations (MBEC 50 ) of host defense peptides toward K. pneumoniae strains

Article Snippet: Specifically, bac7 (1-35) and protegrin-1 displayed greater eradication of a preformed biofilm of strains with hypermucoviscous K1 and K2 capsule serotypes (NTUH K2044 and ATCC 43826) ( and ), than biofilms formed by nonhypermucoviscous strains with K3 and K6 capsule serotypes (ATCC 13883 and ATCC 700603) ( and ).

Techniques: Mutagenesis

Journal: Microbiology Spectrum

Article Title: Polyproline Peptide Aggregation with Klebsiella pneumoniae Extracellular Polysaccharides Exposes Biofilm Associated Bacteria

doi: 10.1128/spectrum.02027-21

Figure Lengend Snippet: Biofilm formation using crystal violet staining. Biofilm formation abilities of K. pneumoniae NTUH K2044 (K1 serotype), 43816 (K2 serotype), ATCC 700603 (K6 serotype), and ATCC 13833 (K3 serotype), transposon parental strain MKP103, and the capsule mutant wza::180T 30 ( wza ). The OD 540 values were corrected for background staining by subtracting the staining of wells with no bacteria. Error between replicates ( n = 6) is shown as ±SEM. K. pneumoniae NTUH was found to have significantly formed more biofilm than all other strains with adjusted P values of <0.0001 for all strains calculated using an ordinary one-way ANOVA with Tukey’s multiple comparisons correction.

Article Snippet: Specifically, bac7 (1-35) and protegrin-1 displayed greater eradication of a preformed biofilm of strains with hypermucoviscous K1 and K2 capsule serotypes (NTUH K2044 and ATCC 43826) ( and ), than biofilms formed by nonhypermucoviscous strains with K3 and K6 capsule serotypes (ATCC 13883 and ATCC 700603) ( and ).

Techniques: Staining, Mutagenesis, Bacteria

Journal: Microbiology Spectrum

Article Title: Polyproline Peptide Aggregation with Klebsiella pneumoniae Extracellular Polysaccharides Exposes Biofilm Associated Bacteria

doi: 10.1128/spectrum.02027-21

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Specifically, bac7 (1-35) and protegrin-1 displayed greater eradication of a preformed biofilm of strains with hypermucoviscous K1 and K2 capsule serotypes (NTUH K2044 and ATCC 43826) ( and ), than biofilms formed by nonhypermucoviscous strains with K3 and K6 capsule serotypes (ATCC 13883 and ATCC 700603) ( and ).

Techniques: Isolation, Mutagenesis

Journal: Frontiers in Pharmacology

Article Title: The Genus Eriosema (Fabaceae): From the Ethnopharmacology to an Evidence-Based Phytotherapeutic Perspective?

doi: 10.3389/fphar.2021.641225

Figure Lengend Snippet: Pharmacological properties of Eriosema spp.

Article Snippet: , E. montanum , Leaves/polar fraction of an EtOH extract , In vitro Bacillus cereus ATCC 14579, Enterobacter cloacae ATCC 13047 Escherichia coli ATCC 8739 Klebsiella pneumoniae ATCC 13883 Mycobacterium fortuitum ATCC 6841 Proteus vulgaris ATCC 13315 Pseudomonas aeruginosa ATCC 15442 Salmonella typhimurium ATCC 13311 Staphylococcus aureus ATCC 6538 Streptococcus pyogenes ATCC 12344 Candida albicans ATCC 10231 Trichophyton rubrum RV 58125, Epidermophyton floccosum RV 71625 Microsporum canis RV 66973 , , MIC > 750 μg/ml , .

Techniques: In Vitro, MTT Assay, Activity Assay, β-carotene Bleaching Assay, DPPH Assay, Concentration Assay, Y2H Assay, In Vivo, Clinical Proteomics, Inhibition, Virus, Infection, Transformation Assay